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記憶形成や学習など大脳の高次機能の分子機構について生化学的、分子生物学的、行動学的解析を主な手技として研究を行っています。

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主な研究実績

奥野浩行

• Takakura M, Nakagawa R, Ota T, Kimura Y, Okuno H, Hirano Y. Rpd3/CoRest-mediated activity-dependent transcription regulates the flexibility in memory updating in Drosophila. Nat Commun, 12:628. (doi: 10.1038/s41467-021-20898-x), 2021.
• Fujita H, Oikawa R, Hayakawa M, Tomoike F, Kimura Y, Okuno H, Hatashita Y, Oliveros CF, Bito H, Ohshima T, Tsuneda S, Abe H, Inoue T. Quantification of native mRNA dynamics in living neurons using fluorescence correlation spectroscopy and reduction-triggered fluorescent probes. J Biol Chem 295:7923-7940, 2020.
• Itoh M, Okuno H, Yamada D, Yamashita M, Abe M, Natsume R, Kaizuka T, Sakimura K, Hoshino M, Mishina M, Wada K, Sekiguchi M, Hayashi T. Perturbed expression pattern of the immediate early gene Arc in the dentate gyrus of GluA1 C-terminal palmitoylation-deficient mice. Neuropsychopharmacol Rep. 9(1):61-66, 2019.
• Yamada M, Suzuki Y, Nagasaki S, Okuno H, Imayoshi I. Light control of the Tet-gene expression system in mammalian cells. Cell Rep, 25:487-500, 2018. 
• Okuno H, Minatohara K, Bito H. Inverse synaptic tagging: An inactive synapse-specific mechanism to capture activity-induced Arc/arg3.1 and to locally regulate spatial distribution of synaptic weights. Semin Cell Dev Biol, 77: 43-50, 2017.
• Minatohara K, Akiyoshi M, Okuno H. Role of immediate-early genes in synaptic plasticity and neuronal ensembles underlying the memory trace. Front Mol Neurosci, 8: Article 78, 2016.
• Vousden DA, Epp J, Okuno H, Nieman BJ, van Eede M, Dazai J, Ragan T, Bito H, Frankland PW, Lerch JP, Henkelman RM: Whole-brain mapping of behaviourally induced neural activation in mice. Brain Struct Funct, 220:2043-2057, 2015.
• Nonaka M, Kim R, Fukushima H, Sasaki K, Suzuki K, Okamura M, Ishii Y, Kawashima T, Kamijo S, Takemoto-Kimura S, Okuno H, Kida S, Bito H: Region-specific activation of CRTC1-CREB signaling mediates long-term fear memory. Neuron, 84:92-106, 2014.
• Kawashima T, Kitamura K, Suzuki K, Nonaka M, Kamijyo S, Takemoto-Kimura S, Kano M, Okuno H, Ohki K, Bito H: Functional labeling of neurons and their projections using the synthetic activity?dependent promoter E-SARE. Nat Methods, 10:889?895, 2013.
• Okuno H, Akashi K, Ishii Y, Yagishita-Kyo N, Suzuki K, Nonaka M, Kawashima T, Fujii H, Takemoto-Kimura S, Abe M, Natsume R, Chowdhury S, Sakimura K, Worley PF, Bito H. Inverse synaptic tagging of inactive synapses via dynamic interaction of Arc/Arg3.1 with CaMKIIβ. Cell, 149:886-898, 2012.
• Kawashima T., Okuno H, Nonaka M, Adachi-Morishima A, Kyo N, Okamura M, Takemoto-Kimura S, Worley P, Bito H: A synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons. Proc Natl Acad Sci USA, 106:316-321, 2009.
• Tokuyama W, Okuno H, Hashimoto T, Li Y-X, Miyashita Y: BDNF upregulation during declarative memory formation in the monkey inferotemporal cortex. Nat Neurosci, 3:1134-1142, 2000. 
• Okuno H., Tokuyama W, Li Y-X, Hashimoto T, Miyashita Y: Quantitative evaluation of neurotrophin and trk mRNA expression in visual and limbic areas along the occipito-temporo-hippocampal pathway in adult macaque monkeys. J Comp Neurol, 408:378-398, 1999.

原口みさこ

• Tabata S, Yamamoto M, Goto H, Hirayama A, Ohishi M, Kuramoto T, Mitsuhashi A, Ikeda R, Haraguchi M, Kawahara K, Shinsato Y, Minami K, Saijo A, Hanibuchi M, Nishioka Y, Sone S, Esumi H, Tomita M, Soga T, Furukawa T, Akiyama SI. Thymidine Catabolism as a Metabolic Strategy for Cancer Survival. Cell Rep. 19(7):1313-1321, 2017.
• Haraguchi M, Sato M, Ozawa M. CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells.PLoS One. 10:e0132260. 2015
• Haraguchi M, Indo HP, Iwasaki Y, Iwashita Y, Fukushige T, Majima HJ, Izumo K, Horiuchi M, Kanekura T, Furukawa T, Ozawa M. Snail modulates cell metabolism in MDCK cells. Biochem Biophys Res Commun. 432:618-625, 2013.
• Haraguchi M. The role of transcriptional regulator snail in cell detachment, reattachment and migration. Cell Adh Migr. 3:259-263, 2009.
• Haraguchi M, Okubo T, Miyashita Y, Miyamoto Y, Hayashi M, Crotti TN, McHugh KP, Ozawa M. Snail regulates cell-matrix adhesion by regulation of the expression of integrins and basement membrane proteins. J Biol Chem. 283(35):23514-23, 2008.
• Haraguchi M, Torii S, Matsuzawa Si, Xie Z, Kitada S, Krajewski S, Yoshida H, Mak TW, Reed JC. Apoptotic protease activating factor 1 (Apaf-1)-independent cell death suppression by Bcl-2. J Exp Med, 191:1709-1720, 2000.
• Haraguchi M, Miyadera K, Uemura K, Sumizawa T, Furukawa T, Yamada K, Akiyama S, Yamada Y. Angiogenic activity of enzymes. Nature, 368:198, 1994.

城山優治

• Goto F, Kiyama* Y, Ogawa I, Okuno H, Ichise T, Ichise H, Anai M, Kodama T, Yoshida N, Bito H, Manabe* T. Gastrin-releasing peptide regulates fear learning under stressed conditions via activation of the amygdalostriatal transition area. Molecular Psychiatry, Accepted for publication, 2021. *, Corresponding authors
• Ohnishi T#, Kiyama Y#, Arima-Yoshida F#, Kadota M#,Ichikawa T, Yamada K, Watanabe A, Ohba H, Tanaka K, Nakaya A, Horiuchi Y, Iwayama Y, Toyoshima M, Ogawa I, Shimamoto-Mitsuyama C, Maekawa M, Balan S, Arai M, Miyashita M, Toriumi K, Nozaki Y, Kurokawa R, Suzuki K, Yoshikawa A, Toyota T, Hosoya T, Okuno H, Bito H, Itokawa M, Kuraku S, Manabe T, Yoshikawa T. Cooperation of LIM domain-binding 2 (LDB2) with EGR in the pathophysiology of schizophrenia. EMBO Mol Med, 13:e12574, 2021. #These authors contributed equally to this work.
• Kiyama Y., Kikkawa Y.S., Kinoshita M., Matsumoto Y., Kondo K., Fujimoto C., Iwasaki S., Yamasoba T., Manabe T. The adhesion molecule cadherin 11 is essential for acquisition of normal hearing ability through middle ear development in the mouse. Laboratory Investigation, doi: 10.1038/s41374-018-0083-y. 2018.
• Nakazawa T., Hashimoto R., Sakoori K., Sugaya Y., Tanimura A., Hashimotodani Y., Ohi K., Yamamori H., Yasuda Y., Umeda-Yano S., Kiyama Y., Konno K., Inoue T., Yokoyama K., Inoue T., Numata S., Ohnuma T., Iwata N., Ozaki N., Hashimoto H., Watanabe M., Manabe T., Yamamoto T., Takeda M. Kano M. Emerging roles of ARHGAP33 in intracellular trafficking of TrkB and pathophysiology of neuropsychiatric disorders. Nature Communications, 7: 10594, 2016.
• Wakabayashi C., Kiyama Y., Kunugi H., Manabe T. Iwakura Y. Age-dependent regulation of depression-like behaviors through modulation of adrenergic receptor alpha(1)A subtype expression revealed by the analysis of interleukin-1 receptor antagonist knockout mice. Neuroscience, 192: 475-484, 2011.
• Morikawa E., Mori H., Kiyama Y., Mishina M., Asano T., Kirino T. Attenuation of focal ischemic brain injury in mice deficient in the epsilon1 (NR2A) subunit of NMDA receptor, Journal of Neuroscience, 18: 9727-9732, 1998.
• Kiyama Y., Manabe T., Sakimura K., Kawakami F., Mori H., Mishina M. Increased thresholds for long-term potentiation and contextual learning in mice lacking the NMDA-type glutamate receptor epsilon1 subunit., Journal of Neuroscience, 18: 6704-6712, 1998.

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奥野浩行

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城山優治

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