• 研究室概要
• 主要研究テーマ
• スタッフ
• 主な研究実績
• 主な研究費取得状況

　当分野では、ヒト感染症の臨床的課題を解決するために、臨床で分離されるさまざまな細菌の病原因子と多様性を明らかにすることを主眼として分子疫学的研究・ゲノム解析の研究を進めています。
　また、医療関連感染の制御を目標とした地域連携組織である鹿児島感染制御ネットワークの事務局として鹿児島県の感染制御レベルの向上に努めるとともに、ワクチンの有効性を明らかにする疫学調査を通じて公衆衛生・予防医学に関わる研究にも取り組んでいます。

研究室概要

連絡先

主要研究テーマ

スタッフ

准教授

研究者総覧 リポジトリ

助教

研究者総覧 リポジトリ

主な研究実績

大岡唯祐

　原著

1. Ooka T, Arai S, Lee K, Gotoh Y, Kubomura A, Imuta N, Hara-Kudo Y, Iyoda S, Hayashi T, and Nishi J. Prevalence, sequence diversity and amplification of an IS-associated enterotoxin gene, astA, in Escherichia coli .  Frontiers in Microbiology, 2025,  16.  in press.
2. Fernandes I, Ooka T, Lira D, Martins FH, Orsi H, Jones N, Elias W, Hayashi T, Gomes TA, and Hernandes R. TccP4: A novel effector identified in the Escherichia albertii strain 1551-2 required for attaching and effacing lesion formation on infected Nck-null cells. Microbiology Spectrum, 2025, Mar 4;13(3):e0205524. doi: 10.1128/spectrum.02055-24.
3. Ooka T, Gotoh Y, Hatanaka S, Yoshimori M, Nishitarumizu K, Kojo K, Kosakamoto H, Sameshima K, Kuroki Y, Chibana N, Doi Y, Yoshino S, Harada T, Seto K, Ikeda T, Miyanohara H, Nakayama K, Gokuden M, Imuta N, Kawamura H, Ogura Y, Hayashi T, Nishi J.A Salmonella enterica serovar Oranienburg clone caused a cluster of bacteremia cases in persons with no recognizable underlying diseases in Japan. Open Forum Infect. Dis, 10:ofac695, 2023, doi: 10.1093/ofid/ofac695.
4. Arai S, Ooka T, Shibata M, Nagai Y, Tokoi Y, Nagaoka H, Maeda R, Tsuchiya A, Kojima Y, Ohya K, Ohnishi T, Konishi N, Ohtsuka K, Hara-Kudo Y. Development of a novel real-time polymerase chain reaction assay to detect Escherichia albertii in chicken meat. Foodborne Pathog. Dis, 19:823-829, 2022. doi: 10.1089/fpd.2022.0042
5. Nakae K, Ooka T, Murakami K, Hara-Kudo Y, Imuta N, Gotoh Y, Ogura Y, Hayashi T, Okamoto Y, Nishi J. Diversification of Escherichia albertii H-antigens and development of H-Genotyping PCR. Front. Microbiol, doi: 10.3389/fmicb.2021.737979. 2021.
6. Ooka T, Seto K, Ogura Y, Nakamura K, Iguchi A, Gotoh Y, Honda M, Etoh Y, Ikeda T, Sugitani W, Konno T, Kawano K, Imuta N, Yoshiie K, Hara-Kudo Y, Murakami K, Hayashi T, Nishi J. O-antigen biosynthesis gene clusters of Escherichia albertii: their diversity and similarity to E. coli gene clusters and the development of an O-genotyping method. Microb. Genom, 5(11). doi: 10.1099/mgen.0.000314, 2019.
7. Masuda K, Ooka T, Akita H, Hiratsuka T, Takao S, Fukada M, Inoue K, Honda M, Toda J, Sugitani W, Narimatsu H, Ishioka T, Hirai S, Sekizuka T, Kuroda M, Morita Y, Hayashi T, Kimura H, Oishi K, Ohnishi M, Fujimoto S, Murakami K. Epidemiological Aspects of Escherichia albertii Outbreaks in Japan and Genetic Characteristics of the Causative Pathogen. Foodborne Pathog. Dis, doi: 10.1089/fpd.2019.2654. 2019.
8. Akter A,#, Ooka T#, Gotoh Y, Yamamoto S, Fujita H, Terasoma F, Kida K, Taira M, Nakadouzono F, Gokuden M, Hirano M, Miyashiro M, Inari K, Shimazu Y, Tabara K, Toyoda A, Yoshimura D, Itoh T, Kitano T, Sato MP, Katsura K, Mondal SI, Ogura Y, Ando S, Hayashi T: Extremely low genomic diversity of Rickettsia japonica distributed in Japan. Genome Biol. Evol, 9:124-133, 2016. (#: These authors contributed equally to this work.)
9. Ooka T, Ogura Y, Katsura K, Seto K, Kobayashi H, Kawano K, Tokuoka E, Furukawa M, Harada S, Yoshino S, Seto J, Ikeda T, Yamaguchi K, Murase K, Gotoh Y, Imuta N, Nishi J, Gomes TA, Beutin L, Hayashi T. Defining the genome features of Escherichia albertii, an emerging enteropathogen closely related to Escherichia coli. Genome Biol Evol 7:3170-3179, 2015
10. Ooka T, Tokuoka E, Furukawa M, Nagamura T, Ogura Y, Arisawa K, Harada S, Hayashi T. Human gastroenteritis outbreak associated with Escherichia albertii, Japan. Emerging infectious diseases 2013;19:144-146
11. Ooka T, Seto K, Kawano K, Kobayashi H, Etoh Y, Ichihara S, Kaneko A, Isobe J, Yamaguchi K, Horikawa K, Gomes TA, Linden A, Bardiau M, Mainil JG, Beutin L, Ogura Y, Hayashi T. Clinical significance of Escherichia albertii. Emerging infectious diseases 2012;18:488-492
12. Ooka T, Terajima J, Kusumoto M, Iguchi A, Kurokawa K, Ogura Y, Asadulghani M, Nakayama K, Murase K, Ohnishi M, Iyoda S, Watanabe H, Hayashi T. Development of a multiplex PCR-based rapid typing method for enterohemorrhagic Escherichia coli O157 strains. Journal of clinical f003-01 2009;47:2888-2894
13. Ooka T, Ogura Y, Asadulghani M, Ohnishi M, Nakayama K, Terajima J, Watanabe H, Hayashi T. Inference of the impact of insertion sequence (IS) elements on bacterial genome diversification through analysis of small-size structural polymorphisms in Escherichia coli O157 genomes. Genome res 2009;19:1809-1816

　総説

1. 大岡唯祐. 新興下痢症起因菌 Escherichia albertiiの特徴. 食品衛生研究 10月号 2020
2. Gomes TA, Ooka T, Hernandes R, Yamamoto D, Hayashi T. Escherichia albertii pathogenesis. EcoSAL plus 9(1): doi: 10.1128/ecosalplus.ESP-0015-2019, 2020
3. 大岡唯祐.　個別菌ゲノム解析（PacBioの利用，annotationやその他の問題点を含む）. 化学療法の領域 33(7):44-50, 2017
4. 大岡唯祐. 新興下痢症原因菌 Escherichia albertii. 日本食品微生物学会誌34(3)：151-157, 2017

　著書

1. 大岡唯祐.（担当：分担執筆）III. 細菌学各論 12. 腸内細菌科, エルシニア科と近縁菌群「総論」、「A. エシェリヒア属」、「B. 赤痢菌属」, 戸田新細菌学（改訂35版, 柳 雄介, 林 哲也, 山﨑 晶 (編)）, 南山堂, 2025.

藺牟田直子

1. Imuta N, Ooka T, Seto K, Kawahara R, Koriyama T, Kojyo T, Iguchi A, Tokuda K, Kawamura H, Yoshiie K, Ogura Y, Hayashi T, Nishi J. Phylogenetic Analysis of Enteroaggregative Escherichia coli (EAEC) Isolates from Japan Reveals Emergence of CTX-M-14-Producing EAEC O25:H4 Clones Related to Sequence Type 131. J Clin Microbiol 54(8):2128-2134, 2016
2. Naito S, Takeuchi N, Ohkusu M, Takahashi-Nakaguchi A, Takahashi H, Imuta N, Nishi J, Shibayama K, Matsuoka M, Sasaki Y, Ishiwada N.　Clinical and Bacteriologic Analysis of Nontypeable Haemophilus influenzae Strains Isolated from Children with Invasive Diseases, Japan, 2008-2015. J Clin Microbiol 56(7), 2018
3. Kanno K, Yamaguchi H, Imuta N, Nishi J, Kasai M. Non-typeable Haemophilus influenzae purulent pericarditis in a healthy child. Pediatr Int 60(9):886-887, 2018
4. Kawamura H, Tokuda K, Imuta N, Kubota T, Koriyama T, Miyanohara H, Hashiguchi T, Kawano Y, Nishi J. Epidemiological Analysis of Nosocomial MRSA Outbreaks Using Phage Open-Reading Frame Typing in a Tertiary-Care Hospital. Jpn J Infect Dis 2016;69(6):523-524
5. Imuta N, Nishi J , Tokuda K, Fujiyama R, Manago K, Iwashita M, Sarantuya J, Kawano Y. Escherichia coli efflux pump TolC promotes aggregation of enteroaggregative E. coli 042. Infect Immun 2008;76(3):1247-1256
6. Wakimoto N, Nishi J, Sheikh J, Nataro JP, Sarantuya J, Iwashita M, Manago K, Tokuda K, Yoshinaga M, Kawano Y. Quantitative biofilm assay using microtiter plate to screen for enteroaggregative Escherichia coli. Am J Trop Med Hyg 2004;71(5):687-690

主な研究費取得状況

大岡唯祐

藺牟田直子

分野サイト