FIELD

Research outline

hip neuron1

Graduate School Graduate School of Medical and Dental Sciences
Course Advanced Therapeutics Course
Field Functional Biology and Pharmacology
Department Biochemistry and Molecular Biology
Homepage https://www.kufm.kagoshima-u.ac.jp/~biochem2/english.html

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Contact

TEL&FAX.+81-99-275-5246

Research interests

  • Regulation of synaptic function by neuronal activity-dependent gene expression
  • Molecular and cellular mechanisms underlying memory-related processes in the brain

Staff

ho 0002

Professor
Name Hiroyuki Okuno
Specialized field Biochemistry、Molecular Biology、Neurobiology, Neurochemistry
Research interests
  • Biological roles of neuronal activity-dependent gene expression
  • Molecular basis of long-term memory

Researche Repository


haraguchi1

Associate Professor
Name Misako HARAGUCHI
Specialized field Molecular Oncology、Cell Biology
Research interests  

Researche Repository


kobayashi1

Assistant Professor
Name Wakako KOBAYASHI
Specialized field Cell Biology、Molecular Biology、Biochemistry
Research interests The analysis of the mechanism of epithelial-mesenchymal transition

Researche Repository

Research results

Hiroyuki Okuno
  • Okuno H, Minatohara K, Bito H. Inverse synaptic tagging: An inactive synapse-specific mechanism to capture activity-induced Arc/arg3.1 and to locally regulate spatial distribution of synaptic weights. Semin Cell Dev Biol, 77: 43-50, 2017.
  • Minatohara K, Akiyoshi M, Okuno H. Role of immediate-early genes in synaptic plasticity and neuronal ensembles underlying the memory trace. Front Mol Neurosci, 8: Article 78, 2016.
  • Vousden DA, Epp J, Okuno H, Nieman BJ, van Eede M, Dazai J, Ragan T, Bito H, Frankland PW, Lerch JP, Henkelman RM: Whole-brain mapping of behaviourally induced neural activation in mice. Brain Struct Funct, 220:2043-2057, 2015.
  • Nonaka M, Kim R, Fukushima H, Sasaki K, Suzuki K, Okamura M, Ishii Y, Kawashima T, Kamijo S, Takemoto-Kimura S, Okuno H, Kida S, Bito H: Region-specific activation of CRTC1-CREB signaling mediates long-term fear memory. Neuron, 84:92-106, 2014.
  • Kawashima T, Kitamura K, Suzuki K, Nonaka M, Kamijyo S, Takemoto-Kimura S, Kano M, Okuno H, Ohki K, Bito H: Functional labeling of neurons and their projections using the synthetic activity?dependent promoter E-SARE. Nat Methods, 10:889?895, 2013.
  • Okuno H, Akashi K, Ishii Y, Yagishita-Kyo N, Suzuki K, Nonaka M, Kawashima T, Fujii H, Takemoto-Kimura S, Abe M, Natsume R, Chowdhury S, Sakimura K, Worley PF, Bito H. Inverse synaptic tagging of inactive synapses via dynamic interaction of Arc/Arg3.1 with CaMKIIβ. Cell, 149:886-898, 2012.
  • Kawashima T., Okuno H, Nonaka M, Adachi-Morishima A, Kyo N, Okamura M, Takemoto-Kimura S, Worley P, Bito H: A synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons. Proc Natl Acad Sci USA, 106:316-321, 2009.
  • Tokuyama W, Okuno H, Hashimoto T, Li Y-X, Miyashita Y: BDNF upregulation during declarative memory formation in the monkey inferotemporal cortex. Nat Neurosci, 3:1134-1142, 2000. 
  • Okuno H., Tokuyama W, Li Y-X, Hashimoto T, Miyashita Y: Quantitative evaluation of neurotrophin and trk mRNA expression in visual and limbic areas along the occipito-temporo-hippocampal pathway in adult macaque monkeys. J Comp Neurol, 408:378-398, 1999.

Misako HARAGUCHI
  • Tabata S, Yamamoto M, Goto H, Hirayama A, Ohishi M, Kuramoto T, Mitsuhashi A, Ikeda R, Haraguchi M, Kawahara K, Shinsato Y, Minami K, Saijo A, Hanibuchi M, Nishioka Y, Sone S, Esumi H, Tomita M, Soga T, Furukawa T, Akiyama SI. Thymidine Catabolism as a Metabolic Strategy for Cancer Survival. Cell Rep. 19(7):1313-1321, 2017.
  • Haraguchi M, Sato M, Ozawa M. CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells.PLoS One. 10:e0132260. 2015
  • Haraguchi M, Indo HP, Iwasaki Y, Iwashita Y, Fukushige T, Majima HJ, Izumo K, Horiuchi M, Kanekura T, Furukawa T, Ozawa M. Snail modulates cell metabolism in MDCK cells. Biochem Biophys Res Commun. 432:618-625, 2013.
  • Haraguchi M. The role of transcriptional regulator snail in cell detachment, reattachment and migration. Cell Adh Migr. 3:259-263, 2009.
  • Haraguchi M, Okubo T, Miyashita Y, Miyamoto Y, Hayashi M, Crotti TN, McHugh KP, Ozawa M. Snail regulates cell-matrix adhesion by regulation of the expression of integrins and basement membrane proteins. J Biol Chem. 283(35):23514-23, 2008.
  • Haraguchi M, Torii S, Matsuzawa Si, Xie Z, Kitada S, Krajewski S, Yoshida H, Mak TW, Reed JC. Apoptotic protease activating factor 1 (Apaf-1)-independent cell death suppression by Bcl-2. J Exp Med, 191:1709-1720, 2000.
  • Haraguchi M, Miyadera K, Uemura K, Sumizawa T, Furukawa T, Yamada K, Akiyama S, Yamada Y. Angiogenic activity of enzymes. Nature, 368:198, 1994.

Wakako KOBAYASHI
  • Tanaka S, Kobayashi W, Haraguchi M, Ishihata K, Nakamura N, Ozawa M. Snail1 expression in human colon cancer DLD-1 cells confers invasive properties without N-cadherin expression. Biochem Biophys Rep, 8:120-126, 2016.
  • Izawa G, Kobayashi W, Haraguchi M, Sudo A, Ozawa M. The ectopic expression of Snail in MDBK cells does not induce epithelial-mesenchymal transition. Int J Mol Med, 36:166-172, 2015.
  • Kobayashi W, Ozawa M. Reversibility of the Snail-induced epithelial-mesenchymal transition revealed by the Cre-loxP system. Biochem Biophys Res Commun, 458:608-613, 2015.
  • Kobayashi W, Ozawa M. Cadherin cytoplasmic domains inhibit the cell surface localization of endogenous E-cadherin, blocking desmosome and tight junction formation and inducing cell dissociation. PLoS One, 9:e105313, 2014.
  • Kobayashi W, Ozawa M. The transcription factor LEF-1 induces an epithelial-mesenchymal transition in MDCK cells independent of β-catenin. Biochem Biophys Res Commun, 442:133-138, 2013.

Patents and copy Rights

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Research Grant List

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