FIELD

Research outline

遺伝子治療・再生医学分野講座スタッフ

Graduate School Graduate School of Medical and Dental Sciences
Course Advanced Therapeutics Course
Field Neuro-musculoskeletal disorder
Department Gene Therapy and Regenerative Medicine
Homepage http://www.kufm.kagoshima-u.ac.jp/~anatomy2/

Homepage

Contact

TEL.+81-99-275-5219 / FAX.+81-99-265-9721

Research interests

  • Development of original vectors and novel gene therapy strategies
  • Developmental biology and regenerative medicine using embryonic stem and iPS cells
  • In vivo regenerative medicine using growth factors and associated molecules
  • Novel angiogenic molecules
  • Epigenetic gene
  • Translational Research

Staff

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Professor
Name Ken-ichiro KOSAI
Specialized field Gene therapy、Regenerative Medicine、Molecular Cell Biology
Research interests
  • 独自ベクターと新規遺伝子治療法の開発
  • ES・iPS 細胞での発生・再生医学
  • 増殖因子と関連分子での生体内再生医学
  • 血管新生抑制分子の解明と応用
  • エピジェネティック遺伝子
  • トランスレーショナル・リサーチ(医師主導治験)

Researche Repository


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Lecturer
Name Kaoru MITSUI
Specialized field Regenerative Medicine、Gene therapy
Research interests 改良型アデノウイルスベクターの多能性幹細胞への応用

Researche Repository


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Assistant Professor
Name Rie IRIE
Specialized field Anatomy、Histology、Histochemistry
Research interests
  • Immunohistochemical analysis of changes in the ultrastructural organization of gastric parietal cells
  • Immunohistochemical analysis of digestive organs
  • 高圧凍結固定・凍結置換法による細胞機能解析

Researche Repository


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Assistant Professor
Name Nobuhiro IJICHI
Specialized field Molecular Cell Biology、Gene therapy
Research interests Gene therapy (Development of vector and therapeutic strategy)

Researche Repository


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特任研究員
Name Kanako IDE
Specialized field Regenerative Medicine、Gene therapy
Research interests 多能性幹細胞の腫瘍化阻止のためのベクターシステムの開発

Researche Repository

Research results

Ken-ichiro KOSAI

総説

  • 小戝健一郎「多因子増殖制御型アデノウイルスの開発と実用化の展望:独自開発の技術を本邦発の革新医薬へ」 実験医学. 34 (1), 19-25, 2016

原著<英文>

  • Mitsui K, Ide K, Takayama A, Wada T, Irie R, Kosai K.: Conditionally replicating adenovirus prevents pluripotent stem cell-derived teratoma by specifically eliminating undifferentiated cells. Mol Ther Methods Clin Dev.  2, 15026; doi:10.1038, 2015 (※Featured article & Editorial)
  • Tanoue K, Wang Y, Ikeda M , Mitsui K, Irie R , Setoguchi T, Komiya S, Natsugoe S, Kosai K.: Survivin-responsive conditionally replicating adenovirus kills rhabdomyosarcoma stem cells more efficiently than their progeny. J Trans Med .12:27.doi: 10.1186 / 1479-5876-12-27, 2014 (※JSGT Meeting Award)
  • Hino S, Sakamoto A, Nagaoka K, Anan K, Wang Y, Mimasu S, Umehara T, Yokoyama S,   Kosai K, and Nakao M.: FAD-dependent lysine-specific demethylase-1 regulates cellular energy expenditure. Nat. Commun. 3:758.doi:10.1038 /ncomms1755,2012
  • Kamisasanuki T, Wang Y, Tokushige S, Terasaki H, Khai NC, Wang Y, Sakamoto T, Kosai K; Targeting CD9 produces stimulus-independent antiangiogenic effects predominantly in activated endothelial cells during angiogenesis: A novel antiangiogenic therapy. Biochem. Biophys. Res. Commun. 413(1): 128-35, 2011
  • Horikawa Y, Wang Y, Nagano S, Kamizono J, Ikeda M, Komiya S, Kosai K.:Assessment of an altered E1B promoter on the specificity and potency of triple-regulated conditionally replicating adenoviruses (CRA) ⎯Implications for the generation of ideal m-CRAs. Cancer Gene Ther 18(10):724-33, 2011
  • Khai NC, Takahashi T, Ushikoshi H, Nagano S, Yuge K, Esaki M, Kawai T, Goto K, Murofushi Y, Fujiwara T, Fujiwara H, Kosai K.: In vivo hepatic HB-EGF gene transduction inhibits Fas-induced liver injury and induces liver regeneration in mice: A comparative study to HGF. J. Hepatol. 44(6): 1046-1054, 2006
  • Kamizono J, Nagano S, Murofushi Y, Komiya S, Fujiwara H, Matsuishi T, Kosai K.: Survivin-responsive conditionally replicating adenovirus exhibits cancer-specific and efficient viral replication. Cancer Res.65(12): 5284-5291, 2005
  • Nagano S, Oshika H, Fujiwara H, Komiya S, Kosai K.: An Efficient Construction of Conditionally Replicating Adenoviruses that Target Tumor Cells with Multiple Factors. Gene Ther .12(18): 1385-1393, 2005
  • Miyado K, Yamada G, Yamada S, Hasuwa H, Nakamura Y, Ryu F, Suzuki K, Kosai K, Inoue K, Ogura A, Okabe M, Mekada E: Requirement of CD9 on the egg plasma membrane for fertilization. Science. 287(5451): 321-324, 2000
  • Kosai K, Matsumoto K, Funakoshi H, Nakamura T.: Hepatocyte growth factor prevents endotoxin-induced lethal hepatic failure in mice. Hepatology. 30(1): 151-159, 1999
  • Chen S-H, Chen XHL, Wang Y, Kosai K, Finegold MJ, Rich S, Woo SL.: Combination gene therapy for liver metastases of colon carcinoma in vivo. Proc. Natl. Acad. Sci. USA.92 (7):2477-2581, 1995

Kaoru MITSUI

原著論文

  • Mitsui K, Ide K, Takayama A, Wada T, Irie R, Kosai K. Conditionally replicating adenovirus prevents pluripotent stem cell-derived teratoma by specifically eliminating undifferentiated cells. Molecular Therapy — Methods & Clinical Development 2, 15026 (2015)(査読有)
  • Tanoue K, Wang Y, Ikeda M, Mitsui K, Irie R, Setoguchi T, Komiya S, Natsugoe S, Kosai K. Survivin-responsive conditionally replicating adenovirus kills rhabdomyosarcoma stem cells more efficiently than their progeny. J Transl Med. 12:27 (2014)(査読有)
  • Mitsui K, Suzuki K, Aizawa E, Kawase E, Suemori H, Nakatsuji N, Mitani K, Gene targeting in human pluripotent stem cells with adeno-associated virus vectors. , Biochem Biophys Res Commun. , Vol.388, No.4, pp.711-717 (2009).
  • Kawasaki T, Saito K, Mitsui K, Ikawa M, Yamashita M, Taniguchi Y, Takeda S, Mitani K, Sakai N., Introduction of a foreign gene into zebrafish and medaka cells using adenoviral vectors., Zebrafish, Vol.6, No.3, pp.253-258 (2009).
  • Suzuki K, Mitsui K, Aizawa E, Hasegawa K, Kawase E, Yamagishi T, Shimizu Y, Suemori H, Nakatsuji N, Mitani K., Highly efficient transient gene expression and gene targeting in primate embryonic stem cells with helper-dependent adenoviral vectors., Proc Natl Acad Sci U S A, Vol.105, No.37, pp.13781-13786 (2008).
  • Tokuzawa Y, Kaiho E, Maruyama M, Takahashi K, Mitsui K, Maeda M, Niwa H, Yamanaka S., Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development., Mol Cell Biol. , Vol.23, No.8, pp.2699-2708 (2003).
  • Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S., The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells., Cell., Vol.113, No.5, pp.631-642 (2003).
  • Takahashi K, Mitsui K, Yamanaka S., Role of ERas in promoting tumour-like properties in mouse embryonic stem cells., Nature., Vol.423, No.6939, pp.541-545 (2003).

Rie IRIE

著書

  • 津山新一郎, 前薗理恵, 村田長芳 高圧凍結法による細胞骨格の動態解析,Acta Anatomica Nipponica vol.80. supplement, 124 (2005)

原著論文

  • Kaoru Mitsui, Kanako , Akiko Takayama, Tadahisa Wada, Rie Irie and Ken-ichiro Kosai Conditionally replicating adenovirus prevents pluripotent stem cell–derived teratoma by specifically eliminating undifferentiated cells. Molecular Therapy — Methods & Clinical Development (2015) 2:15026
  • Tanoue K., Wang Y., Ikeda M., Mitsui K., Irie R., Setoguchi T., Komiya S., Natsugoe S. and Kosai K. Survivin-Responsive Conditionally Replicating Adenovirus Kills Rhabdomyosarcoma Stem Cells More Efficiently Than Their Progeny. Journal of Translational Medicine, 2014, 12(27).
  • Irie-Maezono R. and Tsuyama S. : Immunohistochemical analysis of the acid secretion potency in gastric parietal cells., Open journal of Cell biology., 2013, 2 179-185
  • Murakami K., Kaji T., Shimono R.,  Hayashida Y., Matsufuji H., Tsuyama S., Maezono R., Kosai K., Takamatsu H.. Therapeutic effects of vitamin A on experimental cholestatic rats with hepatic fibrosis., Pediatric Surgery International.27(8) ; 863-70, 2011
  • Wakamatsu D., Tsuyama S., Maezono R., Katoh K., Ogata S., Takao S., Natsugoe S,. Aikoh T. and Murata F..  Immunohistochemical detection of the cytoskeletal components in gastric parietal cells., Acta Histochem. Cytochem. 38(5) ; 331-337, 2005

Nobuhiro IJICHI

著書

  • Ijichi N, Ikeda K, Horie-Inoue K, Inoue S. Roles of Estrogen-Related Receptors in Adipocyte Differentiation. Adipocytes: Biology, Regulation and Health Impact New York: short communication, Chapter 5, 145-154, 2012. Nova Science Publishers.

総説

  • Ijichi N, Ikeda K, Horie-Inoue K, Inoue S. FOXP1 and estrogen signaling in breast cancer. Vitam. Horm. 93:203-12, 2013. doi: 10.1016/B978-0-12-416673-8.00006-X.

原著論文

  • Ijichi N, Shigekawa T, Ikeda K, Miyazaki T, Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki A, Saeki T, Inoue S. Association of Positive EBAG9 Immunoreactivity With Unfavorable Prognosis in Breast Cancer Patients Treated With Tamoxifen. Clin. Breast Cancer 13(6):465-470, 2013. (査読有)
  • Ijichi N, Shigekawa T, Ikeda K, Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki A, Saeki T, Inoue S. Association of double-positive FOXA1 and FOXP1 immunoreactivities with favorable prognosis of tamoxifen-treated breast cancer patients. Horm Cancer 2012; 3: 147-159.(査読有)
  • Shigekawa T, Ijichi N, Ikeda K, Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki A, Saeki T, Inoue S. FOXP1, an estrogen-inducible transcription factor, modulates cell proliferation in breast cancer cells and 5-year recurrence-free survival of patients with tamoxifen-treated breast cancer. Horm Cancer 2011; 2: 286-297.(査読有)
  • Ijichi N, Shigekawa T, Ikeda K, Horie-Inoue K, Fujimura T, Tsuda H, Osaki A, Saeki T, Inoue S. Estrogen-related receptor γ modulates cell proliferation and estrogen signaling in breast cancer. J Steroid Biochem Mol Biol 2011; 123: 1-7.(査読有)
  • Ijichi N, Ikeda K, Fujita M, Usui T, Urano T, Azuma K, Ouchi Y, Horie-Inoue K, and Inoue S: EPAS1, a dexamethasone-inducible gene in osteoblasts, inhibits osteoblastic differentiation. The Open Bone Journal 2009; 1: 28-37.(査読有)
  • Takayama K, Kaneshiro K, Tsutsumi S, Horie-Inoue K, Ikeda K, Urano T, Ijichi N, Ouchi Y, Shirahige K, Aburatani H, and Inoue S: Identification of novel androgen response genes in prostate cancer cells by coupling chromatin immunoprecipitation and genomic microarray analysis. Oncogene 26: 4453-4463, 2007.(査読有)
  • Ijichi N, Ikeda K, Horie-Inoue K, Yagi K, Okazaki Y, and Inoue S: Estrogen-related receptor a modulates the expression of adipogenesis-related genes during adipocyte differentiation. Biochem Biophys Res Commun 2007; 358: 813-818.(査読有)
  • Ijichi N, Tsujimoto N, Iwaki T, Fukumaki Y, Iwaki A. Distal Sox binding elements of the aB-crystallin gene show lens enhancer activity in transgenic mouse embryos. J Biochem 2004; 135: 413-420.(査読有)

Kanako IDE
  • Mitsui K, Ide K, Takayama A, Wada T, Irie R, Kosai K.: Conditionally replicating adenovirus prevents pluripotent stem cell-derived teratoma by specifically eliminating undifferentiated cells. Mol Ther Methods Clin Dev. 2015 Aug 12;2:15026 
  • Furusho Y , Miyata M, Matsuyama T, Nagai T, Li H, Akasaki Y, Hamada N, Miyauchi T, Ikeda Y, Shirasawa T, Ide K, Tei C.Novel therapy for atherosclerosis using recombinant immunotoxin against folatereceptor β-expressing macrophages.(2012) J Am Heart Assoc,Vol.1, pp.e003079-

Patents and copy Rights

各特許の内容についてはお問い合わせください

国内
出願 14題
特許取得 6題

国際

出願 13題
特許取得 米 4題
欧 3題

※JST特許支援事業採択 4題

Research Grant List

Ken-ichiro KOSAI
Project・EventResearchSpan
日本医療研究開発機構研究費(AMED)
(革新的がん医療実用化研究事業)
(研究代表者)Ken-ichiro KOSAI
肉腫への革新医薬実用化を目指した独自開発の増殖制御型アデノウイルスの医師主導治験 平成27
~29年度
日本医療研究開発機構研究費(AMED)
「橋渡し研究加速ネットワークプログラム」研究開発施設共用等促進費補助金 シーズC(京都大学)
(研究代表者)Ken-ichiro KOSAI
独自開発の増殖制御型ウイルス医薬の難治癌への医師主導治験 平成27年度
日本医療研究開発機構研究費(AMED)
「橋渡し研究加速ネットワークプログラム」研究開発施設共用等促進費補助金 シーズA(九州大学)
(研究代表者)Ken-ichiro KOSAI
HB-EGF医薬の難治性疾患への適応拡大による実用化推進 平成27年度
「橋渡し研究加速ネットワークプログラム」研究開発施設共用等促進費補助金 シーズC(京都大学)
(研究代表者)Ken-ichiro KOSAI
独自開発の増殖制御型ウイルス医薬の難治癌への医師主導治験 平成26年度
「橋渡し研究加速ネットワークプログラム」研究開発施設共用等促進費補助金 シーズA(九州大学)
(研究代表者)Ken-ichiro KOSAI
難治性肝疾患を治療するHB-EGF再生医薬の実用化 平成26年度
「橋渡し研究加速ネットワークプログラム」研究開発施設共用等促進費補助金 シーズA(京都大学 / 探索医療研究助成)
(研究代表者)Ken-ichiro KOSAI
難治癌を標的治療する新規の癌溶解性ウイルス医薬の前臨床研究 平成26年度
日本学術振興会 科学研究費補助金
基盤研究(B)
(研究代表者)Ken-ichiro KOSAI
正常と癌の幹細胞の分子機構解明による小児癌への革新的m-CRA治療薬の開発 平成25
~27年度
日本学術振興会 学術研究助成基金助成金 挑戦的萌芽研究
(研究代表者)Ken-ichiro KOSAI
ES/iPS細胞の腫瘍化を克服し再生医療応用を実現するベクター技術の開発 平成25
~26年度
がん対策推進総合研究事業(革新的がん医療実用化研究事業)
※平成26年度より名称変更
難病・がん等の疾患分野の医療の実用化研究事業
(がん関連研究分野)
(研究代表者)Ken-ichiro KOSAI
難治癌を標的治療できる完全オリジナルのウイルス遺伝子医薬の実用化のための前臨床研究 平成24
~26年度
Kaoru MITSUI
Project・Event ResearchSpan
日本学術振興会
科学研究費補助金
基盤研究(C)
(代表研究者)Kaoru MITSUI
ヒトES/iPS細胞由来心筋細胞の腫瘍化克服のための革新的な高純度単離技術の開発 平成24~26年度 
Rie IRIE
Project・EventResearchSpan
日本学術振興会
科学研究費補助金
若手研究(B)
(代表研究者)Rie IRIE
超微形態と独創的実験系によるRett症候群の「非」神経器官の解析による全病態解明 平成26
~28年度
Nobuhiro IJICHI
Project・EventResearchSpan
日本学術振興会
科学研究費補助金
基盤研究(C)
(代表研究者)Nobuhiro IJICHI
独自ベクター技術による癌幹細胞標的・同定と革新的治療の技術開発 平成25
~27年度
Kanako IDE
Project・EventResearchSpan
日本学術振興会
科学研究費補助金
若手研究(B)
(代表研究者)Kanako IDE
ES/iPS細胞の腫瘍化細胞を標的治療可能な革新的ベクターシステムの開発 平成27
~29年度

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